Protein Kinase A, alpha (PRKACA) antibody

Antigen: Protein Kinase A, alpha (PRKACA)

Clonality: Monoclonal (6D2-1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN238374

Additional Information

Characteristics: Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Alternative name: PRKACA

Swiss-Prot: P17612

Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6D2-1

Description: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitoussignaling pathway important in the cell cycle, cellular communication, memory formationand behavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit)and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKArdimer and two activated PKAc ser/thr protein kinase catalytic monomers. The releasedPKAc can translocate into the nucleus and exert a regulatory role in the activation ofmultiple nuclear hormone receptors. However, PKAc-mediated activation oftonicity-dependent gene expression is cAMP independent. Humans express three types ofPKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma aretissue-specific, the later is found in testes.

Specificity: This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit,and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog. Species: Human. Others not tested.

Application Details

Application Notes: Suitable for Western Blotting (0.5-1 μg/ml). Positive control: HeLa human cervix carcinoma cell lineNote: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing thissubunit (testis). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch.

Research Area: Cancer, Kinases/Phosphatases

Restrictions: For Research Use only