Integrin beta 1 (ITGB1) antibody (PE)

Antigen: Integrin beta 1 (ITGB1)

Synonyms: CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA, Fnrb, gpIIa, AA409975, AA960159, 4633401G24Rik, ITGB1, MGC137337, itgb1-B

Clonality: Monoclonal (MEM-101A)

Host: Mouse

Reactivity: Dog (Canine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238444

Additional Information

Alternative name: CD29 / Integrin beta-1

Swiss-Prot: P05556

Immunogen: Raji Burkitt's lymphoma cell line

Cross-Reactivity: Dog (Canine), Human, Pig (Porcine)

Isotype: IgG1

Clone: MEM-101A

Description: CD29 ( b 1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent a chains ( a 1- a 6, CD49a-f) determining the binding properties of b 1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other horseradish peroxidase complex (ABC) as detection reagent. Immunoblotting (1/100-1/1000). Flow Cytometry (1/25-1/200). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most b 1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of b 1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a b 1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1 b . Alternate Names: b 1 integrin subunit, GPIIa.

Specificity: The antibody MEM-101A reacts with CD29 (integrin b 1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin a subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 20 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only