Tubulin, beta (TUBB) antibody

Antigen: Tubulin, beta (TUBB)

Synonyms: M40, TUBB1, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, m40, XLOT, tubb1, tubb5, MGC69508, ok/sw-cl.56, TUBB, TUBB2A, MGC128415, 1t, B1t, BETA 56D, DTB2, Dmbeta1, T, Tub, Tubulin, beta-Tub56D, beta-tub, beta-tubulin56D, beta1, beta1-Tubulin, beta1-tub, beta1Tub, beta1t, beta56D, betaTub1, beta[[1]] tubulin, beta[[1]]-tubulin, DmelCG9277, CG9277, 143391_i_at, 3t, B3t, BETA 60D, D.m.BETA-60D, DTB3, Dmbeta3, beta-Tub60D, beta-Tub6D, beta3, beta3 TU, beta3-Tub, beta3t, beta60C, betaTub3, betaTub60C, beta[[3]] tubulin, beta[[3]]-Tub, beta[[3]]-tubulin, p50, p50/tubulin, p53, p53/tubulin, DmelCG3401, CG3401, 2t, B2t, BETA 85D, BETA2, D.m.BETA-85D, DTB4, Dmbeta2, beta(2)Tu, beta(2)Tub, beta-Tub85D, beta2t, beta2tub, beta85D, betaTub2, beta[[2]]-tubulin, ms(3)KK[D], DmelCG9359, CG9359, 4t, B4t, BETA 98B, beta-Tub, beta-Tub97EF, beta4t, beta97F, betaTub4, betaTub98BC, betaTub98C, CG4869, Dmbeta4, DmelCG4869, DTB1, tubB3, tubulin, Tub1, bmtub1, Tub3, bmtub3, Tub2, bmtub2, Tub4, bmtub4, LOC100101153, TUBB2, dJ40E16.7, MC1R, TUBB4, beta-4, Tubb2, M(beta)2, beta-5, tubb2a, MGC69524, TUBB2C, MGC128183, TUBB3, MGC134115

Clonality: Monoclonal (TU-13)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN238519

Additional Information

Characteristics: Synonyms: Tubulin beta chain, Tubulin beta-5 chain

Alternative name: TUBB / TUBB5

Swiss-Prot: P07437

Immunogen: beta-tubulin from porcine brain.

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine)

Isotype: IgM  (Matching secondary antibodies)

Clone: TU-13

Description: The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number ofmicrotubule-associated proteins (MAPs). The microtubules consist of 13 protofilamentsand have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highlydynamic plus ends and less dynamic minus ends. Microtubules are required for vitalprocesses in eukaryotic cells including mitosis, meiosis, maintenance of cell shape andintracellular transport. Microtubules are also necessary for movement of cells by means offlagella and cilia. In mammalian tissue culture cells microtubules have their minus endsanchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate)molecule is an essential for tubulin heterodimer to associate with other heterodimers toform microtubule. In vivo, microtubule dynamics vary considerably. Microtubulepolymerization is reversible and a populations of microtubules in cells are on their minusends either growing or shortening - this phenomenon is called dynamic instability ofmicrotubules. On a practical level, microtubules can easily be stabilized by the addition ofnon-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugssuch as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Usinglimited proteolysis by enzymes both tubulin subunits can be divided into N-terminal andC-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulinin tubulin heterodimer, it is coded by multiple tubulin genes and it is alsoposttranslationally modified. Heterogeneity of subunit is concentrated in C-terminalstructural domain.

Specificity: The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulinin various species. Species: Human, Porcine, Mouse, Plants. Others not tested.

Synonyms: M40, TUBB1, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, m40, XLOT, tubb1, tubb5, MGC69508, ok/sw-cl.56, TUBB, TUBB2A, MGC128415, 1t, B1t, BETA 56D, DTB2, Dmbeta1, T, Tub, Tubulin, beta-Tub56D, beta-tub, beta-tubulin56D, beta1, beta1-Tubulin, beta1-tub, beta1Tub, beta1t, beta56D, betaTub1, beta[[1]] tubulin, beta[[1]]-tubulin, DmelCG9277, CG9277, 143391_i_at, 3t, B3t, BETA 60D, D.m.BETA-60D, DTB3, Dmbeta3, beta-Tub60D, beta-Tub6D, beta3, beta3 TU, beta3-Tub, beta3t, beta60C, betaTub3, betaTub60C, beta[[3]] tubulin, beta[[3]]-Tub, beta[[3]]-tubulin, p50, p50/tubulin, p53, p53/tubulin, DmelCG3401, CG3401, 2t, B2t, BETA 85D, BETA2, D.m.BETA-85D, DTB4, Dmbeta2, beta(2)Tu, beta(2)Tub, beta-Tub85D, beta2t, beta2tub, beta85D, betaTub2, beta[[2]]-tubulin, ms(3)KK[D], DmelCG9359, CG9359, 4t, B4t, BETA 98B, beta-Tub, beta-Tub97EF, beta4t, beta97F, betaTub4, betaTub98BC, betaTub98C, CG4869, Dmbeta4, DmelCG4869, DTB1, tubB3, tubulin, Tub1, bmtub1, Tub3, bmtub3, Tub2, bmtub2, Tub4, bmtub4, LOC100101153, TUBB2, dJ40E16.7, MC1R, TUBB4, beta-4, Tubb2, M(beta)2, beta-5, tubb2a, MGC69524, TUBB2C, MGC128183, TUBB3, MGC134115

Application Details

Application Notes: Western Blotting (Reducing Conditions): 1 µg/ml, Sonication of MCF7 cells isrecommended. Positive Control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch

Restrictions: For Research Use only