KCNJ11,KATP (Internal Region), (N-Term) antibody

Details for Product No. ABIN238541, Supplier: Log in to see
N-Term, Internal Region
Method Type
Peptide ELISA
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Peptide with sequence C-AEDPAKPRYRARQ, from the internal region (near the N Terminus) of the protein sequence according to NP_000516.3.
Blocking Peptide Blocking peptide for this product available: ABIN368786
Specificity This antibody is expected to recognise isoform 1 (NP_000516.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KCNJ11 / KATP
Background Synonyms: KCNJ11, IKATP, potassium inwardly-rectifying channel, subfamily J, member 11, BIR, HHF2, KIR6.2, MGC133230, PHHI, TNDM3, ATP-sensitive inward rectifier potassium channel 11, beta-cell inward rectifier subunit, inwardly rectifying potassium channel KIR6.2, potassium inwardly-rectifying channel J11
Gene ID 3767
NCBI Accession NP_000516
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45-48 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.5 kDa according to NP_000516.3). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-KCNJ11,KATP (Internal Region), (N-Term) antibody (ABIN238541) ABIN238541 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). ...
Background publications Filosa, Bonev, Straub, Meredith, Wilkerson, Aldrich, Nelson: "Local potassium signaling couples neuronal activity to vasodilation in the brain." in: Nature neuroscience, Vol. 9, Issue 11, pp. 1397-1403, 2006 (PubMed).

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