Chromodomain Helicase DNA Binding Protein 7 (CHD7) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 7 (CHD7)

Synonyms: IS3, KAL5, FLJ20357, FLJ20361, KIAA1416, Dz, Mt, Cyn, Edy, Flo, Lda, Obt, Whi, Cycn, Todo, WBE1, A730019I05Rik, RP23-464N23.1, CHD7, fd19h06, KIAA0308, wu:fb37f10, wu:fb39h04, wu:fd19h06, wu:cegs2051, si:ch211-197o6.2

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238550

Additional Information

Alternative name: CHD7

Gene ID: 55636, 320790 (mouse), 312974 (rat)

Immunogen: C-KDEIDEFANSPSE

Format: Affinity-purified

Description: All names and symbols: chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3. Official Symbol: CHD7. RefSeq number(s): NP_060250.2

Specificity: Human, Mouse, Rat

Synonyms: IS3, KAL5, FLJ20357, FLJ20361, KIAA1416, Dz, Mt, Cyn, Edy, Flo, Lda, Obt, Whi, Cycn, Todo, WBE1, A730019I05Rik, RP23-464N23.1, CHD7, fd19h06, KIAA0308, wu:fb37f10, wu:fb39h04, wu:fd19h06, wu:cegs2051, si:ch211-197o6.2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336kDa according to Human NP_060250.2 and 175kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Gao, Gordon, Zhang et al.: "CHD7 gene polymorphisms are associated with susceptibility to idiopathic scoliosis." in: American journal of human genetics, Vol. 80, Issue 5, pp. 957-65, 2007 (PubMed).