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DIAPH3 antibody (Diaphanous Homolog 3 (Drosophila)) (C-Term)

Details for Product anti-DIAPH3 Antibody No. ABIN238601, Supplier: Log in to see
Antigen
  • 4930417P13Rik
  • AN
  • AUNA1
  • DIA2
  • Dia2
  • Diap3
  • diap3
  • DIAP3
  • Diaph3
  • DIAPH3
  • Drf3
  • DRF3
  • mDia2
  • mKIAA4117
  • NSDAN
  • p134MDia2
Epitope
C-Term, Internal Region
26
16
5
4
3
2
2
2
2
1
1
1
Reactivity
Human
56
6
6
Host
Goat
38
13
6
Clonality
Polyclonal
Conjugate
This DIAPH3 antibody is un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
43
34
5
3
2
1
1
Supplier
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Immunogen Peptide with sequence C-RTPMPKDVRQS, from the internal region (near the C terminus) of the protein sequence according to NP_001035982.1, NP_112194.2.
Blocking Peptide Blocking peptide for this product available: ABIN369835
Specificity This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DIAPH3 (DIAPH3 Antibody Abstract)
Background Synonyms: DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3
Gene ID 81624
NCBI Accession NP_112194, NP_001035982
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 50 kDa band in human bone marrow, liver and lymph node lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137 kDa according to NP_001035982.1 and 98.1 kDa according to NP_112194.2. The ~50 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Yang, Czech, Gerboth, Kojima, Scita, Svitkina: "Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells." in: PLoS biology, Vol. 5, Issue 11, pp. e317, 2007 (PubMed).