APOBEC3A antibody (Internal Region)
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- Target See all APOBEC3A Antibodies
- APOBEC3A (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This APOBEC3A antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Phorbolin 1 / APOBEC3A
- Sequence
- TSNFNNGIGR HKTY
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-TSNFNNGIGRHKTY, from the internal region of the protein sequence according to NP_663745.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6 μg/mL.
Western Blot: Approx. 26-28 kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0 kDa according to Human NP_663745.1). This molecular weight is routinely observed by other sources. Recommended concent
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- APOBEC3A (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A))
- Alternative Name
- APOBEC3A (APOBEC3A Products)
- Synonyms
- A3A antibody, ARP3 antibody, PHRBN antibody, bK150C2.1 antibody, APOBEC3A antibody, APOBEC3Z1 antibody, apolipoprotein B mRNA editing enzyme catalytic subunit 3A antibody, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A antibody, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3Z1 antibody, similar to ARP10 protein antibody, APOBEC3A antibody, APOBEC3Z1 antibody
- Background
- APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1
- Gene ID
- 200315
- NCBI Accession
- NP_663745
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