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Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (Internal Region) antibody

Details for Product No. ABIN238659, Supplier: Log in to see
Antigen
  • BC056494
  • MARCH-IV
  • mKIAA1399
  • RNF174
Epitope
Internal Region
24
15
10
3
2
2
1
1
1
Reactivity
Human
65
24
21
6
4
3
3
2
2
2
2
1
1
Host
Goat
53
13
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
36
33
12
11
9
1
Supplier
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Immunogen Peptide with sequence DFCKEKTEDRYS, from the internal region of the protein sequence according to NP_065865.1.
Blocking Peptide Blocking peptide for this product available: ABIN370076
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MARCH-IV (MARCH4 Antibody Abstract)
Background Synonyms: MARCH4, membrane-associated ring finger (C3HC4) 4, MARCH-IV, MGC104908, RNF174, membrane-associated RING-CH protein IV
Gene ID 57574
NCBI Accession NP_065865
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Bartee, Mansouri, Hovey Nerenberg, Gouveia, Früh: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

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