Cullin 4A antibody

Catalog No. ABIN2425716

Product Details anti-Cullin 4A Antibody

Target: Cullin 4A (CUL4A)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human CUL4A

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid freeze / thaw cycles.

Storage: -20 °C/-80 °C

Storage Comment: Store at -20°C (regular) and -80°C (long term).

Target Details for Cullin 4A

Target: Cullin 4A (CUL4A)

Alternative Name: CUL4A (CUL4A Products)

Synonyms: zgc:64071 antibody, CUL4A antibody, 2810470J21Rik antibody, AW495282 antibody, RGD1563853 antibody, cullin 4A antibody, CUL4A antibody, cul4a antibody, Cul4a antibody

Background: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.

Molecular Weight: Calculated MW: 88 kDa