ETF1 antibody
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- Target See all ETF1 Antibodies
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ETF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Characteristics
- Homo sapiens eukaryotic translation termination factor 1 (ETF1)
- Purification
- Purified from mouse ascites fluids by affinity chromatography
- Immunogen
- Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
- Clone
- 2E3
- Isotype
- IgG2a
- Top Product
- Discover our top product ETF1 Primary Antibody
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- Application Notes
- WB 1:500, FLOW 1:100,
- Comment
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The concentration of the product may vary between diferrent lots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5-1.0 mg/mL
- Buffer
- PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
- Alternative Name
- ETF1 (ETF1 Products)
- Synonyms
- D5S1995 antibody, ERF antibody, ERF1 antibody, RF1 antibody, SUP45L1 antibody, TB3-1 antibody, AI463371 antibody, D6Ertd109e antibody, eRF1 antibody, zeh0057 antibody, hm:zeh0057 antibody, wu:fa19e07 antibody, wu:fi31f01 antibody, cl1 antibody, RRF1 antibody, DDBDRAFT_0188023 antibody, DDBDRAFT_0191343 antibody, DDB_0188023 antibody, DDB_0191343 antibody, CG5605 antibody, Dm0184 antibody, Dmel\\CG5605 antibody, Group J antibody, group J antibody, l(3)00103 antibody, l(3)05637 antibody, l(3)77ABb antibody, l(3)neo28 antibody, Eukaryotic release factor 1 antibody, eukaryotic translation termination factor 1 antibody, eukaryotic translation termination factor 1b antibody, eukaryotic translation termination factor 1 S homeolog antibody, eukaryotic peptide chain release factor subunit 1 antibody, eukaryotic petide chain release factor subunit 1 antibody, eukaryotic release factor 1 antibody, Eukaryotic peptide chain release factor subunit 1 antibody, ETF1 antibody, Etf1 antibody, etf1b antibody, etf1 antibody, etf1.S antibody, Tb11.22.0012 antibody, THAPSDRAFT_22240 antibody, TTHERM_00292170 antibody, LOC692937 antibody, erf1 antibody, eRF1 antibody, erfa-1 antibody
- Background
- Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
- Molecular Weight
- 48.9 kDa
- Gene ID
- 2107
- NCBI Accession
- NM_004730
- HGNC
- 2107
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