NDUFA5 antibody (AA 3-116)

Catalog No. ABIN2726996

Product Details anti-NDUFA5 Antibody

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Binding Specificity: AA 3-116

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NDUFA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13 kDa (NDUFA5), nuclear gene encoding mitochondrial protein

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Human recombinant protein fragment corresponding to amino acids 3-116 of human NDUFA5(NP_004991) produced in HEK293T cell.

Clone: 1E8

Isotype: IgG1

Application Details

Application Notes: WB 1:2000, IF 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for NDUFA5

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Alternative Name: NDUFA5 (NDUFA5 Products)

Synonyms: NADHUO antibody, B13 antibody, CI-13KD-B antibody, CI-13kB antibody, NUFM antibody, UQOR13 antibody, 2900002J19Rik antibody, NADH:ubiquinone oxidoreductase subunit A5 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 antibody, Ndufa5 antibody, NDUFA5 antibody

Background: The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.

Molecular Weight: 13.3 kDa

Gene ID: 4698

NCBI Accession: NM_005000

HGNC: 4698