ATP2B3 antibody

Catalog No. ABIN2458840

Product Details anti-ATP2B3 Antibody

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2B3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP2B3.

Application Details

Application Notes: ATP2B3 antibody can be used for detection of ATP2B3 by ELISA at 1:1562500. ATP2B3 antibody can be used for detection of ATP2B3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATP2B3 antibody at -20 °C.

Target Details for ATP2B3

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Alternative Name: ATP2B3 (ATP2B3 Products)

Synonyms: PMCA3 antibody, PMCA3a antibody, SCAX1 antibody, atp2b3 antibody, pmca3a antibody, zgc:92885 antibody, 6430519O13Rik antibody, Pmca3 antibody, ATPase plasma membrane Ca2+ transporting 3 antibody, ATPase, Ca++ transporting, plasma membrane 3a antibody, ATPase, Ca++ transporting, plasma membrane 3 antibody, plasma membrane calcium-transporting ATPase 2 antibody, ATP2B3 antibody, atp2b3a antibody, Atp2b3 antibody, atp2b3 antibody, LOC100639471 antibody

Background: ATP2B3 gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. ATP2B3 is the plasma membrane calcium ATPase isoform 3.The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 134 kDa

Gene ID: 492

NCBI Accession: NP_001001344

UniProt: Q16720

Pathways: Ribonucleoside Biosynthetic Process