POLR3F antibody

Catalog No. ABIN2459222

Product Details anti-POLR3F Antibody

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3F antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3F.

Application Details

Application Notes: POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR3F antibody at -20 °C.

Target Details for POLR3F

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Alternative Name: POLR3F (POLR3F Products)

Synonyms: 2810411G20Rik antibody, 3010019O03Rik antibody, 3110032A07Rik antibody, RPC39 antibody, RPC6 antibody, zgc:56299 antibody, zgc:76983 antibody, RNA polymerase III subunit F antibody, polymerase (RNA) III (DNA directed) polypeptide F antibody, POLR3F antibody, Polr3f antibody, polr3f antibody

Background: POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.

Molecular Weight: 36 kDa

Gene ID: 10621

NCBI Accession: NP_006457

UniProt: Q9H1D9