MED21 antibody

Catalog No. ABIN2461591

Product Details anti-MED21 Antibody

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED21.

Application Details

Application Notes: MED21 antibody can be used for detection of MED21 by ELISA at 1:62500. MED21 antibody can be used for detection of MED21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MED21 antibody at -20 °C.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: MED21 (MED21 Products)

Synonyms: SURB7 antibody, fk48d02 antibody, surb7 antibody, wu:fk48d02 antibody, zgc:56532 antibody, SRB7 antibody, hSrb7 antibody, 0610007L03Rik antibody, AI449604 antibody, D19234 antibody, D6Ertd782e antibody, Srb7 antibody, Surb7 antibody, T4B21.8 antibody, T4B21_8 antibody, mediator 21 antibody, mediator complex subunit 21 antibody, Med21p antibody, mediator 21 antibody, MED21 antibody, med21 antibody, Med21 antibody

Background: MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Molecular Weight: 15 kDa

Gene ID: 9412

NCBI Accession: NP_004255

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha