U2AF2 antibody

Catalog No. ABIN2462083

Product Details anti-U2AF2 Antibody

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human U2AF2.

Application Details

Application Notes: U2AF2 antibody can be used for detection of U2AF2 by ELISA at 1:62500. U2AF2 antibody can be used for detection of U2AF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store U2AF2 antibody at -20 °C.

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF2 (U2AF59 Products)

Synonyms: U2AF65 antibody, 65kDa antibody, u2af65 antibody, U2AF2 antibody, DDBDRAFT_0186947 antibody, DDBDRAFT_0233353 antibody, DDB_0186947 antibody, DDB_0233353 antibody, zgc:110115 antibody, U2 small nuclear RNA auxiliary factor 2 antibody, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 antibody, U2 small nuclear RNA auxiliary factor 2 S homeolog antibody, RNA recognition motif-containing protein RRM antibody, Splicing factor U2AF 65 kDa subunit antibody, U2 small nuclear RNA auxiliary factor 2a antibody, U2AF2 antibody, U2af2 antibody, u2af2.S antibody, u2af2 antibody, u2af2a antibody

Background: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Molecular Weight: 52 kDa, 54 kDa

Gene ID: 11338

NCBI Accession: NP_001012496

UniProt: Q96HC5