HNRNPF antibody

Catalog No. ABIN2462177

Product Details anti-HNRNPF Antibody

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNRPF.

Application Details

Application Notes: HNRPF antibody can be used for detection of HNRPF by ELISA at 1:62500. HNRPF antibody can be used for detection of HNRPF by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HNRPF antibody at -20 °C.

Target Details for HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Alternative Name: HNRPF (HNRNPF Products)

Synonyms: HNRPF antibody, OK/SW-cl.23 antibody, mcs94-1 antibody, HNRNPF antibody, Hnrpf antibody, 4833420I20Rik antibody, AA407306 antibody, QtsA-10715 antibody, hnRNP F antibody, heterogeneous nuclear ribonucleoprotein F antibody, HNRNPF antibody, Hnrnpf antibody

Background: HNRPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.

Molecular Weight: 46 kDa

Gene ID: 3185

NCBI Accession: NP_004957

UniProt: P52597