SF3A1 antibody

Catalog No. ABIN2462195

Product Details anti-SF3A1 Antibody

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3A1.

Application Details

Application Notes: SF3A1 antibody can be used for detection of SF3A1 by ELISA at 1:62500. SF3A1 antibody can be used for detection of SF3A1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SF3A1 antibody at -20 °C.

Target Details for SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Alternative Name: SF3A1 (SF3A1 Products)

Synonyms: Dmel\\CG16941 antibody, SF3a1 antibody, SF3a120 antibody, DDBDRAFT_0168938 antibody, DDBDRAFT_0233180 antibody, DDB_0168938 antibody, DDB_0233180 antibody, 1200014H24Rik antibody, 5930416L09Rik antibody, AI159724 antibody, si:dz150f13.2 antibody, wu:fc38e01 antibody, wu:fc50a11 antibody, wu:fj37c05 antibody, zgc:65786 antibody, PRP21 antibody, PRPF21 antibody, SAP114 antibody, SF3A120 antibody, CG16941 gene product from transcript CG16941-RA antibody, SWAP/Surp domain-containing protein antibody, splicing factor 3a, subunit 1 antibody, splicing factor 3a subunit 1 antibody, CG16941 antibody, sf3a1 antibody, spl1 antibody, Sf3a1 antibody, SF3A1 antibody

Background: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 89 kDa

Gene ID: 10291

NCBI Accession: NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization