MRPL24 antibody

Catalog No. ABIN2462302

Product Details anti-MRPL24 Antibody

Target: MRPL24 (Mitochondrial Ribosomal Protein L24 (MRPL24))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL24 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MRPL24.

Application Details

Application Notes: MRPL24 antibody can be used for detection of MRPL24 by ELISA at 1:62500. MRPL24 antibody can be used for detection of MRPL24 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MRPL24 antibody at -20 °C.

Target Details for MRPL24

Target: MRPL24 (Mitochondrial Ribosomal Protein L24 (MRPL24))

Alternative Name: MRPL24 (MRPL24 Products)

Synonyms: L24mt antibody, MRP-L18 antibody, MRP-L24 antibody, CG8849 antibody, Dmel\\CG8849 antibody, L24 antibody, RpL24 antibody, 2010005E08Rik antibody, 2810470K06Rik antibody, 6720473G22Rik antibody, AA407670 antibody, rpl24 antibody, rpl24-A antibody, GB10626 antibody, zgc:92702 antibody, l24mt antibody, mrp-l18 antibody, mrp-l24 antibody, mitochondrial ribosomal protein L24 antibody, mitochondrial ribosomal protein L24 L homeolog antibody, probable 39S ribosomal protein L24, mitochondrial antibody, mitochondrial 54S ribosomal protein YmL24/YmL14 antibody, mitochondrial ribosomal protein subunit L28 (predicted) antibody, Putative mitochondrial ribosomal protein L24 antibody, MRPL24 antibody, mRpL24 antibody, Mrpl24 antibody, mrpl24 antibody, mrpl24.L antibody, LOC408675 antibody, CAALFM_C203950WA antibody

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL24 is a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24).Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein.

Molecular Weight: 25 kDa

Gene ID: 79590

NCBI Accession: NP_078816

UniProt: Q96A35