NXF5 antibody

Catalog No. ABIN2462318

Product Details anti-NXF5 Antibody

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NXF5.

Application Details

Application Notes: NXF5 antibody can be used for detection of NXF5 by ELISA at 1:312500. NXF5 antibody can be used for detection of NXF5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NXF5 antibody at -20 °C.

Target Details for NXF5

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Alternative Name: NXF5 (NXF5 Products)

Synonyms: NXF2B antibody, nuclear RNA export factor 5 antibody, nuclear RNA export factor 2 antibody, NXF5 antibody, LOC609849 antibody, Nxf5 antibody

Background: NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene.

Molecular Weight: 44 kDa, 72 kDa, 72 kDa, 72 kDa, 72 kDa, 72 kDa

Gene ID: 55998

NCBI Accession: NP_116564

UniProt: Q9H1B4