NXF5 antibody (Middle Region)

Catalog No. ABIN2776599

The Rabbit Polyclonal anti-NXF5 antibody is suitable to detect NXF5 in samples from Human, Dog, Mouse, Rabbit and Rat. It has been validated for WB and IHC.

Quick Overview for NXF5 antibody (Middle Region) (ABIN2776599)

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Reactivity: Human, Dog, Mouse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NXF5 Antibody

Binding Specificity: Middle Region

Purpose: NXF5 Antibody - middle region

Sequence: ITERNFPELL SLNLCNNKLY QLDGLSDITE KAPKVKTLNL SKNKLESAWE

Predicted Reactivity: Dog: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against NXF5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NXF5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106879

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NXF5

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Alternative Name: NXF5

Background:

Background Information: NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene.

Gene Name: Nuclear RNA export factor 5

Protein Name: Nuclear RNA export factor 5

Molecular Weight: 44kDa

Gene ID: 55998

NCBI Accession: NP_116564

UniProt: Q9H1B4