MKRN1 antibody

Catalog No. ABIN2462668

Product Details anti-MKRN1 Antibody

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Reactivity: Human, Mouse, Dog, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKRN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MKRN1.

Application Details

Application Notes: MKRN1 antibody can be used for detection of MKRN1 by ELISA at 1:312500. MKRN1 antibody can be used for detection of MKRN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MKRN1 antibody at -20 °C.

Target Details for MKRN1

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Alternative Name: MKRN1 (MKRN1 Products)

Synonyms: MKRN1 antibody, MGC84269 antibody, mkrn1 antibody, Makorin-1 antibody, RNF61 antibody, RFP antibody, makorin ring finger protein 1 antibody, E3 ubiquitin-protein ligase makorin-1 antibody, makorin ring finger protein 1 S homeolog antibody, makorin, ring finger protein, 1 antibody, MKRN1 antibody, LOC100393879 antibody, mkrn1.S antibody, Mkrn1 antibody

Background: The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Molecular Weight: 16 kDa, 17 kDa

Gene ID: 23608

NCBI Accession: NP_038474

UniProt: Q9UHC7

Pathways: Chromatin Binding