SLC25A14
Reactivity: Rat
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
SLC25A14 antibody can be used for detection of SLC25A14 by ELISA at 1:62500. SLC25A14 antibody can be used for detection of SLC25A14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store SLC25A14 antibody at -20 °C.
Target
SLC25A14
(Solute Carrier Family 25 (Mitochondrial Carrier, Brain), Member 14 (SLC25A14))
ATPUMP5 antibody, DIC1 antibody, DICARBOXYLATE CARRIER 1 antibody, F14M13.10 antibody, F14M13_10 antibody, PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 5 antibody, uncoupling protein 5 antibody, zgc:55596 antibody, BMCP1 antibody, UCP5 antibody, BMCP-1 antibody, Ucp5 antibody, UCP5L antibody, UCP5S antibody, Bmcp1 antibody, solute carrier family 25 member 14 antibody, uncoupling protein 5 antibody, solute carrier family 25 (mitochondrial carrier, brain), member 14 antibody, SLC25A14 antibody, UCP5 antibody, slc25a14 antibody, Slc25a14 antibody
Background
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. SLC25A14 has an N-terminal hydrophobic domain that is not present in other UCPs.