p107 antibody

Catalog No. ABIN2463962

Product Details anti-p107 Antibody

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p107 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBL1.

Application Details

Application Notes: RBL1 antibody can be used for detection of RBL1 by ELISA at 1:62500. RBL1 antibody can be used for detection of RBL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RBL1 antibody at -20 °C.

Target Details for p107

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Alternative Name: RBL1 (RBL1 Products)

Synonyms: p107 antibody, prb1 antibody, fj58e06 antibody, wu:fj58e06 antibody, CP107 antibody, PRB1 antibody, AW547426 antibody, retinoblastoma-like 1 L homeolog antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma-like 1 (p107) antibody, retinoblastoma-like 1 antibody, rbl1.L antibody, RBL1 antibody, rbl1 antibody, Rbl1 antibody

Background: RBL1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 115 kDa

Gene ID: 5933

NCBI Accession: NP_899662

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases