CAMK2A antibody (Internal Region)

Catalog No. ABIN2464145

Product Details anti-CAMK2A Antibody

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).

Purification: CAMK2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CAMK2A antibody was raised against a 14 amino acid synthetic peptide near the internal region of CAMK2A.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 55+65 kDa bands observed in human cerebellum and mouse brain lysates (calculated MW of 55.3 kDa according to human NP_057065.2 and of 54.1 according to human NP_741960.1 and mouse NP_803126.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human brain cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration, 3-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A (CAMK2A Products)

Synonyms: camk2 antibody, camk2b antibody, zgc:112538 antibody, zgc:123320 antibody, CAMKA antibody, CaMKII antibody, R74975 antibody, mKIAA0968 antibody, PK2CDD antibody, PKCCD antibody, calcium/calmodulin dependent protein kinase II alpha antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin-dependent protein kinase II alpha antibody, CAMK2A antibody, camk2a.S antibody, camk2a antibody, Camk2a antibody

Gene ID: 815

NCBI Accession: NP_057065, NP_741960

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction