Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
PPP4C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PPP4C antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of PPP4C.
PPP4C
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 35 kDa band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1 kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.