ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Purification
PEBP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PEBP4 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of PEBP4.
PEBP4
Reactivity: Human
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band observed in human heart lysates (calculated MW of 25.7 kDa according to NP_659399.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
PEBP4
(Phosphatidylethanolamine-Binding Protein 4 (PEBP4))