Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
FOXI3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FOXI3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of FOXI3.
FOXI3
Reactivity: Human
WB, ELISA, FACS
Host: Rabbit
Polyclonal
FITC
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 50 kDa band in human skin lysates after 1 μg/mL antibody staining . The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Bronchus shows strong nuclear staining in the basal cells of the resporatory epithelium. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.