This antibody is expected to recognize isoform 1 (NP_005091.2) only.
Purification
Akap12 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Akap12 antibody was raised against a amino acid synthetic peptide near the internal region of Akap12 (mouse).
AKAP12
Reactivity: Rat, Cow
WB, IHC, IP
Host: Sheep
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments in NIH3T3 lysate and of mouse, rat brain, and testis lysates gave no specific signal but low background (at antibody concentration up to 2 μg/mL).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
AKAP12
(A Kinase (PRKA) Anchor Protein 12 (AKAP12))