Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms (NP_057631.1, NP_057260.2).
Purification
SDF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SDF4 antibody was raised against a 15 amino acid synthetic peptide near the internal region of SDF4 (aa 161-175).
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 45 kDa band observed in Jurkat lysate (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. IHC: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.