SDF4 antibody (AA 161-175)

Catalog No. ABIN625824

Product Details anti-SDF4 Antibody

Target: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Binding Specificity: AA 161-175

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SDF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: SDF4 (aa161-175)

Sequence: EYKVKFLASK GHSEK

Specificity: This antibody is expected to recognize both reported isoforms NP_057631.2, and NP_057260.3

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: CEYKVKFLASKGHSEK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SDF4

Target: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Alternative Name: SDF4 (SDF4 Products)

Synonyms: Cab45 antibody, SDF-4 antibody, MGC107861 antibody, wu:fb66d06 antibody, wu:fd46e11 antibody, zgc:56577 antibody, zgc:76916 antibody, CAB45 antibody, stromal cell derived factor 4 antibody, stromal cell derived factor 4 L homeolog antibody, SDF4 antibody, sdf4 antibody, sdf4.L antibody, Sdf4 antibody

Background: SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4

Gene ID: 51150, 20318, 155173

NCBI Accession: NP_057631, NP_057260