This antibody is expected to recognize both reported isoforms (NP_001668.1, NP_001001787.1).
Purification
ATP1B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ATP1B1 antibody was raised against a 15 amino acid synthetic peptide near the internal region of ATP1B1.
ATP1B1
Reactivity: Human, Mouse, Rat
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 48 kDa band observed in rat retina lysates and 50 kDa in mouse and rat kidney lysates (calculated MW of 35.1 kDa according to human NP_001668.1, 35.2 kDa according to mouse NP_033851.1 and rat NP_037245.2).The observed molecular weight corresponds to the glycosylated form (PMID: 20130120). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.