MYO5A antibody (Internal Region)

Catalog No. ABIN2464899

Product Details anti-MYO5A Antibody

Target: MYO5A (Myosin VA (MYO5A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MYO5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: This antibody is expected to recognize both reported isoforms (NP_000250.3, NP_001135967.1).

Purification: Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: MYO5A antibody was raised against a 10 amino acid synthetic peptide near the internal region of MYO5A.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 200 kDa band observed in Jurkat lysate (calculated MW of 215 kDa according to NP_000250.3). Recommended concentration: 1-3 μg/mL. Immunofluorescence: Transverse sections of mouse skeletal muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (R der et al, Proc Natl Acad Sci U S A. 2010 Feb 2,107 (5) :2031-6, PMID: 20133847). Recommended concentration: 1-3 3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Alternative Name: MYO5A (MYO5A Products)

Synonyms: myo5a antibody, MGC53270 antibody, si:dkey-266j9.1 antibody, MYOA antibody, MYO5A antibody, D antibody, Dop antibody, Myh12 antibody, 9630007J19Rik antibody, AI413174 antibody, AI661011 antibody, Dbv antibody, MVa antibody, Myo5 antibody, MyoVA antibody, Sev-1 antibody, d antibody, d-120J antibody, flail antibody, flr antibody, DILUTE antibody, myoxin) antibody, non-muscle antibody, GS1 antibody, MYH12 antibody, MYO5 antibody, MYR12 antibody, myosin VA L homeolog antibody, myosin VAa antibody, myosin VA antibody, myosin va antibody, myo5a.L antibody, myo5aa antibody, MYO5A antibody, PY04789 antibody, Myo5a antibody

Gene ID: 4644

NCBI Accession: NP_000250, NP_001135967

UniProt: Q9Y4I1

Pathways: Hormone Transport, Peptide Hormone Metabolism