This antibody is expected to recognize isoform a (NP_006278.1) only.
Purification
TFPI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TFPI antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of TFPI.
TFPI
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 50 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0 kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approximately 38 kDa to 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.