Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
4E-T antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
4E-T antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of 4E-T.
EIF4ENIF1
Reactivity: Human, Mouse, Rat, Pig, Cow, Guinea Pig, Horse, Bat, Hamster, Monkey
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution >1/32,000. Western Blot: Approximately 140-150 kDa band observed in 293 lysates (predicted size of approximately 108 kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below). Recommended for use at 0.25-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas shows strong staining of cytoplasm in acinar cells. Recommended concentration 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.