ANILLIN antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ANILLIN antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ANILLIN.
ANLN
Reactivity: Human, Mouse
FACS, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 555
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 75 kDa band in mouse brain lysates after 0.3 μg/mL antibody staining.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration, 3-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
Anillin (ANLN)
(Anillin, Actin Binding Protein (ANLN))