This antibody is expected to recognize isoform 1 (NP_079088.1) only.
Purification
BAALC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
BAALC antibody was raised against a 15 amino acid synthetic peptide near the internal region of BAALC.
BAALC
Reactivity: Human, Mouse, Rat
WB, IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 488
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 18 kDa band observed in mouse brain lysates (calculated MW of 15.6 kDa according to human NP_079088.1 and of 15.5 kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa in both human and mouse brain lysates. This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
BAALC
(Brain and Acute Leukemia, Cytoplasmic (BAALC))