CARD6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CARD6 antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of CARD6.
Verified
CARD6
Reactivity: Human
ELISA, IHC
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 45-50 kDa and 25-30 kDa bands observed in A549, human heart and human muscle lysates at 1 ? of primary. Please note that the bands we observe are different from the predicted size of approximately 119 kDa according to NP_115976. Immunohistochemistry: In paraffin embedded human pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
CARD6
(Caspase Recruitment Domain Family, Member 6 (CARD6))