This alpha-Mannosidase II antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Purification
MAN2A1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MAN2A1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of MAN2A1.
MAN2A1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approximately 98 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
alpha-Mannosidase II (MAN2A1)
(Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1))