Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
MEPCE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MEPCE antibody was raised against a 13 amino acid synthetic peptide near the internal region of MEPCE.
MEPCE
Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Pig
WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa according to human NP_062552.2 and 75.3 kDa according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approximately 75 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. IHC: In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.