This antibody is expected to recognize isoform 1 (NP_055099.1, NP_001014839.1) and isoform 2 (NP_001014841.1).
Purification
Neurochondrin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Neurochondrin antibody was raised against a 14 amino acid synthetic peptide near the internal region of Neurochondrin.
NCDN
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 100 kDa and140 kDa in human cerebellum lysates after 1 μg/mL antibody staining.9 kDa according to NP_055099.1 and 77.2 kDa according to NP_001014841.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.