This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).
Purification
P4HA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
P4HA1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of P4HA1.
P4HA1
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0 kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
P4HA1
(Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))