Pallidin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Pallidin antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Pallidin.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 23-24 kDa band observed in lysates of cell line A431 (calculated MW of 19.7 kDa according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approximately 28 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.