RAB11FIP1 antibody (Internal Region)

Catalog No. ABIN2466172

Product Details anti-RAB11FIP1 Antibody

Target: RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAB11FIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is expected to recognize isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1).

Purification: RAB11FIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: RAB11FIP1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of RAB11FIP1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 110 kDa, 50 kDa and 38 kDa in human breast lysate after 1 μg/mL antibody staining.0 kDa according to NP_079427.3, and 137 kDa according to NP_001002814.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Please note that an additional 55 kDa was only partly blocked by the immunizing peptide, this band may not be specific. Approximately 80 kDa band observed in HeLa lysate (calculated MW of 71.0 kDa according to NP_079427.3). The observed molecular weight of 80 kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5,277(14):12190-9. PMID 11786538.). Recommended concentration: 0.1-0.3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for RAB11FIP1

Target: RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))

Alternative Name: RAB11FIP1 (RAB11FIP1 Products)

Synonyms: zgc:122989 antibody, NOEL1A antibody, RCP antibody, rab11-FIP1 antibody, 2010200K21Rik antibody, 4833414G05Rik antibody, Rcp antibody, RGD1562965 antibody, Rab11-FIP1 antibody, RAB11 family interacting protein 1 antibody, RAB11 family interacting protein 1 (class I) a antibody, RAB11 family interacting protein 1 (class I) antibody, RAB11FIP1 antibody, rab11fip1a antibody, Rab11fip1 antibody

Gene ID: 80223

NCBI Accession: NP_079427, NP_001002814

UniProt: Q6WKZ4