Semaphorin 5A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Semaphorin 5A antibody was raised against a 14 amino acid synthetic peptide near the internal region of Semaphorin 5A.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 35 kDa band in human hippocampus lysates after 1 μg/mL antibody staining.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human prostate shows cytoplasm staining of secretory cells in the glands. Recommended concentration, 1.25 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows membanous and vesicular staining in PCT cells. Recommended concentration, 4-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SEMA5A
(Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))