This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
Purification
WISP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
WISP1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of WISP1.
WISP1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Western Blot: In transfected HEK293 transiently expressing WISP1 a band of approximately 35 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))