Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms according to NP_569079.1 and NP_064520.2.
Purification
WRNIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
WRNIP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of WRNIP1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 45 kDa band in human lung and brain lysates after 1 μg/mL antibody staining.2 and 69.5 kDa for isoform 2 according to NP_569079.1. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 90 kDa band observed in Jurkat lysate. an additional band of unknown identity was also consistently observed at 50 kDa . This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
WRNIP1
(Werner Helicase Interacting Protein 1 (WRNIP1))