LIM Domain Binding 3 Protein antibody (Internal Region)

Catalog No. ABIN2466462

Product Details anti-LIM Domain Binding 3 Protein Antibody

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 3 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: ZASP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: ZASP antibody was raised against a 12 amino acid synthetic peptide near the internal region of ZASP.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Experiments gave bands at approximately 80-90 kDa and 30-35 kDa in human muscle lysates after 0.01 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28,278 (9) :7360-5, PMID: 12499364). This protein has a calculated MW of 77.1 kDa according to NP_009009.1. Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human skeletal muscle shows staining of the Z lines in the fibres. Recommended concentration, 3-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for LIM Domain Binding 3 Protein

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Alternative Name: ZASP (LDB3 Products)

Synonyms: CMD1C antibody, CYPHER antibody, LDB3Z1 antibody, LDB3Z4 antibody, LVNC3 antibody, MFM4 antibody, ORACLE antibody, PDLIM6 antibody, ZASP antibody, AW742271 antibody, ldb3l antibody, zgc:55814 antibody, wu:fi31d08 antibody, ldb3 antibody, MGC52706 antibody, MGC75668 antibody, RGD1564875 antibody, LDB3 antibody, cypher antibody, hm:zehn1639 antibody, zehn1639 antibody, LIM domain binding 3 antibody, LIM domain binding 3b antibody, LIM domain binding 3 S homeolog antibody, LIM domain binding 3a antibody, LDB3 antibody, Ldb3 antibody, ldb3b antibody, ldb3.S antibody, ldb3 antibody, ldb3a antibody

Gene ID: 11155

NCBI Accession: NP_009009

UniProt: O75112