ZCCHC11 antibody (C-Term)

Catalog No. ABIN2466463

Product Details anti-ZCCHC11 Antibody

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZCCHC11 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize all 3 reported isoforms (as represented by NP_056084.1, NP_001009882.1, NP_001009881.1)

Purification: ZCCHC11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: ZCCHC11 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ZCCHC11.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Immunohistochemistry: In paraffin embedded tissues recommended concentration, 2-6 μg/mL. Western Blot: Approximately 250-300 kDa and 30-35 kDa band seen in Raji lysate. Recommended for use at 1-3 μg/mL.

Please note that the band we consistently detect in Western Blots differs from the predicted size of 181 kDa . However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for ZCCHC11

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Alternative Name: ZCCHC11 (ZCCHC11 Products)

Synonyms: PAPD3 antibody, TUT4 antibody, ZCCHC11 antibody, DKFZp459G145 antibody, 6030404K05Rik antibody, 9230115F04Rik antibody, PPAPD3 antibody, mKIAA0191 antibody, wu:fc06h08 antibody, wu:fy37g11 antibody, papd3 antibody, zinc finger CCHC-type containing 11 antibody, zinc finger, CCHC domain containing 11 antibody, zinc finger CCHC domain containing 11 antibody, ZCCHC11 antibody, Zcchc11 antibody, zcchc11 antibody

Gene ID: 23318

NCBI Accession: NP_056084, NP_001009882, NP_001009881

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance