Western Blotting (WB), ELISA, Flow Cytometry (FACS)
Purification
ABCB5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ABCB5 antibody was raised against a 13 amino acid synthetic peptide near the internal region of ABCB5.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 80 kDa band observed in lysates of A431. Recommended concentration: 0.03-0.1 μg/mL. Approximately 90 kDa band observed in human skin lysates and in lysates of cell line A431 (calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ABCB5
(ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))