This antibody is expected to recognize both reported isoforms (NP_006539.3 and NP_001007226.1).
Purification
IGF2BP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
IGF2BP2 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of IGF2BP2.
IGF2BP2
Reactivity: Human
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 60-70 kDa band observed in HEPG2 lysate (calculated MW of 66.1 kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approximately 65 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
IGF2BP2
(Insulin-Like Growth Factor 2 mRNA Binding Protein 2 (IGF2BP2))